"N-glycan structural analysis have a complicated workflow. Typically, liquid chromatography (LC) coupled to fluorescent detection (FLR) will be used to produce and analyze fluorescently labeled N-glycans, LC with detection usually use mass spectrometry (MS), or capillary electrophoresis (CE) with laser-induced fluorescence (LIF). Nowadays, within the pharmaceutical industry, there is no single standardized protocol for N-glycan analysis while most organizations have developed customized workflows to suit their individual needs. The N-glycan fingerprint of membrane proteins can be obtained by using DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE). MALDI ion trap analysis of plasma N-glycans documents features that discriminate between primary and secondary causes of under glycosylation and should be applied as the first step in the diagnostic track with an unsolved CDG type II of all patients.
Creative Proteomics have a strict workflow to analyze N-Glycans to meet your requirements."